human stem cell nucleofecto kit Search Results


human stem cell nucleofecto kit  (Lonza)
90
Lonza human stem cell nucleofecto kit
Human Stem Cell Nucleofecto Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stem cell nucleofecto kit/product/Lonza
Average 90 stars, based on 1 article reviews
human stem cell nucleofecto kit - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
amaxa human stem cell nucleofector kit 1  (Lonza)
90
Lonza amaxa human stem cell nucleofector kit 1
Amaxa Human Stem Cell Nucleofector Kit 1, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amaxa human stem cell nucleofector kit 1/product/Lonza
Average 90 stars, based on 1 article reviews
amaxa human stem cell nucleofector kit 1 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
human stem cell nucleofection buffer  (Lonza)
90
Lonza human stem cell nucleofection buffer
Human Stem Cell Nucleofection Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stem cell nucleofection buffer/product/Lonza
Average 90 stars, based on 1 article reviews
human stem cell nucleofection buffer - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
human stem cell nucleofector kit 1  (Amaxa)
90
Amaxa human stem cell nucleofector kit 1
Human Stem Cell Nucleofector Kit 1, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stem cell nucleofector kit 1/product/Amaxa
Average 90 stars, based on 1 article reviews
human stem cell nucleofector kit 1 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
nucleofection hesc 497 solution 1  (Lonza)
90
Lonza nucleofection hesc 497 solution 1
Nucleofection Hesc 497 Solution 1, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nucleofection hesc 497 solution 1/product/Lonza
Average 90 stars, based on 1 article reviews
nucleofection hesc 497 solution 1 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
pmaxgfp  (Lonza)
90
Lonza pmaxgfp
hTERT MSCs were transfected with either <t>pmaxGFP</t> (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.
Pmaxgfp, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pmaxgfp/product/Lonza
Average 90 stars, based on 1 article reviews
pmaxgfp - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
amaxa nucleofector  (Amaxa)
90
Amaxa amaxa nucleofector
hTERT MSCs were transfected with either <t>pmaxGFP</t> (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.
Amaxa Nucleofector, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amaxa nucleofector/product/Amaxa
Average 90 stars, based on 1 article reviews
amaxa nucleofector - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
4d-nucleofector® basic protocol for human stem cells  (Amaxa)
90
Amaxa 4d-nucleofector® basic protocol for human stem cells
hTERT MSCs were transfected with either <t>pmaxGFP</t> (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.
4d Nucleofector® Basic Protocol For Human Stem Cells, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4d-nucleofector® basic protocol for human stem cells/product/Amaxa
Average 90 stars, based on 1 article reviews
4d-nucleofector® basic protocol for human stem cells - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
human stem cell nucleofector kit 2  (Lonza)
96
Lonza human stem cell nucleofector kit 2
hTERT MSCs were transfected with either <t>pmaxGFP</t> (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.
Human Stem Cell Nucleofector Kit 2, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stem cell nucleofector kit 2/product/Lonza
Average 96 stars, based on 1 article reviews
human stem cell nucleofector kit 2 - by Bioz Stars, 2026-04
96/100 stars
  Buy from Supplier
human stem cell nucleofector solution  (Eppendorf AG)
90
Eppendorf AG human stem cell nucleofector solution
hTERT MSCs were transfected with either <t>pmaxGFP</t> (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.
Human Stem Cell Nucleofector Solution, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human stem cell nucleofector solution/product/Eppendorf AG
Average 90 stars, based on 1 article reviews
human stem cell nucleofector solution - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
amaxa human stem cell nucleofector  (Lonza)
90
Lonza amaxa human stem cell nucleofector
hTERT MSCs were transfected with either <t>pmaxGFP</t> (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.
Amaxa Human Stem Cell Nucleofector, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/amaxa human stem cell nucleofector/product/Lonza
Average 90 stars, based on 1 article reviews
amaxa human stem cell nucleofector - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
hmsc nucleofector  (Lonza)
99
Lonza hmsc nucleofector
hTERT MSCs were transfected with either <t>pmaxGFP</t> (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.
Hmsc Nucleofector, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hmsc nucleofector/product/Lonza
Average 99 stars, based on 1 article reviews
hmsc nucleofector - by Bioz Stars, 2026-04
99/100 stars
  Buy from Supplier

Image Search Results


hTERT MSCs were transfected with either pmaxGFP (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.

Journal: bioRxiv

Article Title: Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and emulsion coupling assay as a low-cellularity workflow for single-cell cloning

doi: 10.1101/2020.08.17.253559

Figure Lengend Snippet: hTERT MSCs were transfected with either pmaxGFP (transfection efficiency 61%) or pNV-RANKL/KO (transfection efficiency 6%). Two days post-transfection, GFP expressing single-cells were isolated by SCP (see details below). In (A) the SCP (f.sight™) cartridge ejection area is recorded during the printing process, showing a fluorescent single-cell before (01-03), at (04), and after (05) ejection. The SCP algorithm intercepts and deflects cells that are not qualifying the preset parameters (size threshold was set from 15 – 30 μ m, roundness from 0.6 – 1, where 1 reflects the perfectly circular object). In (B) and (D) the roundness is plotted against cell diameter and in (C) and (E) the relative fluorescence intensity unit (RFU) is plotted against cell diameter - all detected events are in gray. The red dots represent printed single-cells qualified to the preset parameters (see below). The dotted orange line represents the thresholds of parameters for selecting single cells. (F) The printed single-cells were evaluated for clonality (n= 451 transfected printed cells). The recorded image series of each qualified printing process was evaluated manually, categorizing single, multiple-cell, uncertain, or void printing events. (G) After 2 weeks colonies obtained from single cells were counted and thereby the cloning efficiency determined (n=227 transfected printed cells, n=650 non-transfected printed cells). The single-cell printing parameters applied for pmaxGFP transfected hTERT MSCs: laser intensity of 20 - 40%, an exposure time of 15 - 30 ms and a fluorescence threshold of 30 - 250 RFU while for pNV-RANKL/KO transfected hTERT MSCs laser intensity of 90-95%, exposure time of 80 - 120 ms and a fluorescence threshold of 50 - 250 RFU.

Article Snippet: Three vectors were purchased with the following gRNA sequences: pNV-RANKL1/KO - 5’-CAGGAATTACAACATATCGT-3’ (472221110290), pNV-RANKL2/KO - 5’-CAGCGATGGTGGATGGCTCA-3’ (472221110390), pNV-RANKL3/KO - 5’-TTAATAGTGAGATGAGCAAA-3’ (472221110490). pmaxGFP (purchased from Lonza, Human MSC Nucleofector ® Kit) was used to control transfection efficiency.

Techniques: Transfection, Expressing, Isolation, Fluorescence, Clone Assay